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E-Catalog > อุปกรณ์และเครื่องมือ ค้นหาและวิเคราะห์โปรตีน > Bradford Red Assay
Product Information :
- เครื่องมือวิทยาศาสตร์ / สารเคมีและวัสดุภัณฑ์สำหรับห้องปฏิบัติการ
- อุปกรณ์และเครื่องมือ ค้นหาและวิเคราะห์โปรตีน
- สินค้า, ผลิตภัณฑ์
Product Detail :
BradfordRed
Benefits:
- Enhanced linearity
- Greater range and concentration of detergent compatibility
- Reducing agent compatible
- Only 10ul sample required
- Fast
- Convenient room temperature storage
Standard curves for BSA using BradfordRed and competitor B Bradford assay. Absorbances were measured at 660nm for the BradfordRed assay and 595nm for the competitor Bradford assay. BradfordRed has a greater linear range (25 to 2000µg) than the competitor Bradford assay (125 to 1000µg).
BradfordRed is based on the binding of a dye-metal complex to protein in acidic conditions that causes a shift in the dye's absorption maximum, which is measured at 660nm. The colour produced is stable and increases in proportion to a broad range of increasing protein concentrations, even in the presence of detergents and reducing agents that would be incompatible with standard Bradford and BCA protein assays. The ionic detergent compatibility (IDCR) reagent may be added to the assay reagent to increase compatibility with high amounts of ionic detergents, to measure samples containing Laemmli SDS sample buffer with bromophenol blue.
